![]() ![]() ![]() We confirmed the MUTZ- 3 response to DNCB by real-time PCR analysis. Of these markers, CD86 proved to be the most reliable since it detected all sensitizers, including benzocaine, a classical false negative in local lymph node assay (LLNA) but not irritants. Then, we tested MUTZ- 3 cells against a wider panel of sensitizers and irritants on a broader spectrum of cell surface markers (HLA-DR, CD40, CD54, CD80, CD86, B7-H 1, B7-H2, B7-DC). Only MUTZ- 3 and THP- 1 cells show a strong and specific response to sensitizer, while other cell lines showed very variable responses. First, we compared undifferentiated MUTZ- 3 cells with several standard human cells such as THP- 1, KG- 1, HL-60, K-562, and U- 937 in their response to the strong sensitizer DNCB and the irritant SDS by monitoring the expression levels of HLA-DR, CD54, and CD86 by flow cytometry. In this study, we have evaluated the performances of MUTZ- 3, a cytokine-dependent human monocytic cell line, in its response to sensitizers. However, no in vitro methods exist which are based on the activation process of LC to predict the sensitization potential of chemicals. Langerhans cells (LC) are key mediators of contact allergenicity in the skin. International Nuclear Information System (INIS)Īzam, Philippe Peiffer, Jean-Luc Chamousset, Delphine Tissier, Marie-Helene Bonnet, Pierre-Antoine Vian, Laurence Fabre, Isabelle Ourlin, Jean-Claude You can only upload a photo or a video.The cytokine-dependent MUTZ- 3 cell line as an in vitro model for the screening of contact sensitizers.You can only upload a photo (png, jpg, jpeg) or a video (3gp, 3gpp, mp4, mov, avi, mpg, mpeg, rm).You can only upload videos smaller than 600MB.You can only upload photos smaller than 5 MB.You can only upload files of type 3GP, 3GPP, MP4, MOV, AVI, MPG, MPEG, or RM.You can only upload files of type PNG, JPG, or JPEG.We are experiencing some problems, please try again.Please upload a file larger than 100x100 pixels Perangkat: LG TV Model: 29UF3RL Masalah: Mati total, tidak ada power dan B+ short Penyebab: capa. Transistor yang biasa digunakan untuk penguat akhir sebuah modul power amplifier 1. ![]()
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